PRSS11; HTRA; IGFBP5-protease; L56; HtrA; ORF480; HtrA1, a 51kD protein, is a member of highly conserved mammalian HtrA serine protease family.
It is a human homolog of bacterial HtrA and consists of a secretory signal sequence, an IGFBP-binding domain and a Kazal-type serine-protease inhibitor domain at the N-terminus and a highly conserved trypsin-like serine:protease domain and a PDZ domain at the C-terminus.
It plays a major role in the regulation of cell growth.
HtrA1 inhibits signaling mediated by TGF-beta family members and regulates many physiological processes.
HtrA1 is up-regulated in placenta during pregnancy and plays an important role in implantation and placental physiology.
It regulates the availability of IGFs by cleaving IGF-binding proteins.
It acts as a tumor suppressor and is down-regulated in ovarian cancers and melanomas.
Its role has been implicated in osteoarthritis suggesting a therapeutic intervention.
Northern blot analysis detected high expression in placenta.
Applications:Suitable for use in Immunofluorescence/Immunocytochemistry and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:250-1:1000Immunofluorescence (IC): 1:10-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum.
Form：Supplied as a liquid, 0.025% sodium azide, 50% glycerol.
Specificity：Recognizes human HtrA1. Species Crossreactivity: chimpanzee, bovine, canine, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IC IF WB
Calc Crossreactivity：Bo Ca Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa234-262 of human HtrA1.
