HOX homeobox genes play a role both in the early stem cell function as well as in later stages of hematopoietic differentiation.
HOXB4, specifically, is a homeotic selector gene implicated in self-renewal of definitive HSCs.
Expression of HOXB4 in primitive progenitors combined with culture on hematopoietic stroma induced a switch to the definitive HSC phenotype.
Activation of WNT signaling in HSCs induced increased expression of HOXB4.
Perturbations of HOX gene expression can cause leukemia.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1-3ug/mlImmunohistochemistry (formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human HOXB4. Species Crossreactivity: feline, chimpanzee, bovine, mouse, rat. Species sequence homology: chicken, gorilla, equine, opossum, porcine, rhesus monkey, xenopus.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Bo Fe Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa100-150 of human HOXB4.
