HIPK3, a mammalian homolog of YAK1, is a novel 130kD nuclear co-factor that belongs to the HIPK protein family.
Members of this family localize to nuclear speckles and consist of an evolutionarily conserved protein kinase domain and a homeoprotein-interacting domain.
Physiological functions of HIPK3 have been predicted in different cell signaling pathways including transcriptional co-repression, cell differentiation, growth suppression, steroidogenesis and apoptosis.
Research suggests that HIPK3 is a FADD-interacting protein kinase found to phosphorylate FADD and promote its binding to Fas, thereby inhibiting Fas-mediated Jun NH (2)-terminal kinase activation.
HIPK3 also has been found to phosphorylate JNK and c-JUN, thus enhancing SF-1 activity for CYP11A1 transcription.
HIPK3 plays a key role in cAMP-dependent signaling pathway.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/mlImmunohistochemistry (formalin fixed paraffin embedded): 5-10ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Testis
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity：Recognizes human HIPK3. Species Crossreactivity: chimpanzee, canine, mouse, rat.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Ca Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa850-900 of human HIPK3.
