HDAC-9 is a histone deacetylase with an important role in the regulation of gene expression and hematopoiesis.
A nuclear protein, HDAC-9 belongs to the histone deacetylase family with several isoforms and is inhibited by Trichostatin A (TSA) and suberoylanilide hydroxamic acid.
It helps in deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) and gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events.
MEF2-dependent transcription is repressed by HDAC-9 by recruiting HDAC-1 and/or HDAC-3.
It also inhibits skeletal myogenesis and is involved in heart development.
Ubiquitous but variable expression is seen in brain and other tissues like heart, pancreas and also in human bladder carcinoma cells.
Lack of HDAC-9 protein is found in a family with Peters anomaly, a central corneal leukoma.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/mlImmunohistochemistry (formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human HDAC9. Species Crossreactivity: chimpanzee, bovine, canine, equine, mouse, opossum. Species sequence homology: chicken.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Bo Ca Eq Hu Mo
Immunogen：Synthetic peptide corresponding to aa100-150 of human HDAC-9.
