84-1192-42　GUK1 (Guanylate Kinase, GMP Kinase, GMK) 100ul　G9803-40C

特徴

• Guanylate kinase ([EC:2.7.4.8]) (GK) catalyzes the ATP-dependent phosphorylation of GMP into GDP.
• It is essential for recycling GMP and indirectly, cGMP.
• In prokaryotes (such as Escherichia coli), lower eukaryotes (such as yeast) and in vertebrates, GK is a highly conserved monomeric protein of about 200aa.
• GK has been shown to be structurally similar to protein A57R (or SalG2R) from various strains of Vaccinia virus.
• Proteins containing one or more copies of the DHR domain, an SH3 domain as well as a C-terminal GK-like domain, are collectively termed MAGUKs (membrane-associated guanylate kinase homologs) and include Drosophila lethal(1)discs large-1 tumor suppressor protein (gene dlg1); mammalian tight junction protein Zo-1; a family of mammalian synaptic proteins that seem to interact with the cytoplasmic tail of NMDA receptor subunits (SAP90/PSD-95, CHAPSYN-110/PSD-93, SAP97/DLG1 and SAP102); vertebrate 55 kD erythrocyte membrane protein (p55); C.
• elegans protein lin-2; rat protein CASK; and human proteins DLG2 and DLG3.
• There is an ATP-binding site (P-loop) in the N-terminal section of GK, which is not conserved in the GK-like domain of the above proteins.
• However these proteins retain the residues known, in GK, to be involved in the binding of MP.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:250-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.