GRK5 is a 590aa protein belonging to the G protein-coupled receptor kinase family and consisting of AGC-kinase C-terminal, protein kinase and RGS domains.
It phosphorylates the activated forms of G protein-coupled receptors, thus initiating their deactivation.
It also phosphorylates the human substance P receptor and plays a role in regulating the motility of polymorphonuclear leukocytes (PMNs).
It contains a membrane binding motif consisting of a group of hydrophobic amino acids that mediates its plasma membrane (PM) localization.
GRK5 is expressed in heart, placenta, lung, skeletal muscle, brain, liver, pancreas and kidney.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-4ug/mlImmunohistochemistry (paraffin): 20ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity：Recognizes human GRK5. Species Crossreactivity: bovine, mouse, rat. Species sequence homology: chicken, pufferfish, zebrafish.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Bo Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa350-400 of human GRK5.
