GNPDA1 is an allosteric enzyme catalyzing the reversible conversion of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium, a reaction that under physiological conditions proceeds to the formation of fructose-6-phosphate.
It is the sole enzyme linking hexosamine systems with glycolytic pathways by transferring hexosamines from structural macromolecules into the glycolytic pathway.
GNPDA1 localizes close to the developing acrosome vesicle and in spermatozoa close to the acrosomal region, and may play a role in the acrosome reaction.
Human GNPDA1 gene is localized to chromosome 5q21.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ug/mlImmunohistochemistry (formalin fixed paraffin embedded): 10ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Human, mouse and rat spleen lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human GNPDA1. Species Crossreactivity: mouse and rat. Species sequence homology: bovine.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa100-150 of human GNPDA1.
