GLI1 is a protein originally isolated in human glioblastoma.
The GLI proteins are the effectors of Sonic Hedgehog (Hh) signaling and have been shown to be involved in cell-fate determination, proliferation and patterning in many cell types and most organs during embryo development.
GLI1 was originally isolated from a glioma tumor and has been found to be up regulated in many tumors including muscle, brain and skin tumors such as Basal cell carcinoma (BCC).
Shh and the Gli genes are normally expressed in hair follicles, and skin tumors expressing Gli1 may arise from hair follicles.
The level of Gli1 expression correlates with the tumor grade in bone and soft tissue sarcomas.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1-3ug/mlImmunohistochemistry (Formalin fixed paraffin): 5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity：Recognizes human GLI1. Species Crossreactivity: rhesus monkey. Species Sequence Homology: equine (94%), canine (88%), bovine, mouse and rat (76%)
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to a portion of aa940-990 from human GLI1.
