G6B is a 241aa novel regulatory nuclear chloride ion channel protein belonging to the immunoglobulin (Ig) super family.
It is a glycosylated receptor, present both as soluble and transmembrane isoforms where the soluble proteins show expression in endoplasmic reticulum and Golgi before being secreted.
It contains a leader sequence and an extracellular Ig domain which binds to heparin and helps to modulate the interaction of G6B with an unknown protein ligand.
It also has an intracellular, proline-rich potential SH3-binding domain, and two putative phosphorylated tyrosine motifs with an SH2-binding domain that is recruited to the receptor and dephosphorylates membrane-bound phosphoproteins and thus modulates the signaling cascade.
It is commonly expressed in certain hematopoietic cell lines including K562, Molt4, and Jurkat.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:K562 cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity：Recognizes human G6B. Species Crossreactivity: chimpanzee. Species sequence homology: mouse, rat, bovine, canine, monkey.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa1-50 of human G6B.
