84-1192-05　［取扱停止］FAP-1 (Tyrosine-protein Phosphatase Non-receptor Type 13, Fas-associated Protein-tyrosine Phosphatase 1, PTP-BAS, Protein-tyrosine Phosphatase 1E, PTP-E1, hPTPE1, Protein-tyrosine Phosphatase PTPL1, PTPN13, PNP1, PTP1E, PTPL1) 50ul　F0019-53N4

特徴

• FAP-1 (also known as Fas-associated phosphatase-1, PTPN13, PTP-BAS, hPTPIE, and PTPL1) is a member of the protein tyrosine phosphatase (PTP) family.
• PTPs are enzymes that catalyze the removal of a phosphate group attached to a tyrosine residue.
• Most intracellular signaling involves reversible phosphorylation events; therefore, PTPs are central to the dynamic regulation of signaling cascades that underlie cell functions.
• For example, PTPs play key roles in regulating cell growth, differentiation, proliferation, inflammation, and oncogenic transformation.
• PTPs are emerging as a promising class of signaling targets for diseases such as cancer, neurodegeneration, diabetes, and inflammation.
• A key challenge is to identify specific PTPs that are invovled in a disease process and develop therapeutics to modulate the PTP.
• FAP-1 phosphatase is thought to be important in the Fas signaling pathway.
• FAP-1 binds to the cytosolic tail of the Fas receptor (Apo1, CD95) and inhibits Fas-induced apoptosis.
• Increased FAP-1 protein levels in some tumor cell lines and tumor tissues correlates with resistance to Fas-mediated apoptosis.
• In general, FAP-1 expression has been found to be highest in cell lines and tissues that are relatively resistant to Fas-mediated apoptosis.
• Gene transfer-mediated elevations in FAP-1 partially abolished Fas-induced apoptosis in a T cell line which is consistent with an inhibitory effect of FAP-1 on Fas signal transductions.
• Additionally, FAP-1 expression correlates with Fas resistant in ovarian cancer cell lines and FAP-1 is commonly expression in ovarian cancers.
• Human Fas has a putative consensus tyrosine phosphorylation site (Tyrosine 275) suggesting that Fas surface expression or signaling may be regulated by phosphorylation.
• FAP-1 has been shown to directly bind to Fas and may dephosphorylate Fas as part of the down regulation of the apoptotic pathway.
• It is thought that development of therapeutics to inhibit FAP-1 may increase the ability of tumor cells with upregulated FAP-1 to undergo apoptosis.
• FAP-1 is a large ~270kD protein.
• Multiple alternatively spliced FAP-1 transcript variants which encode distinct proteins have been reported, including shorter forms.
• Please see Application Notes section for additional details on FAP-1 isoforms.
• Recognizes FAP-1, including FAP-1 isoforms listed in the Application Notes section.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:2000Immunohistochemistry (formalin fixed paraffin embedded): 1:1000-1:5000Immunoprecipitation: 1:50-1:200Immunohistochemistry: FrozenOptimal dilutions to be determined by the researcher.
• Positive Control:Spleen, lymphoid, many cancer cell lines
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.