eIF4B is a multidomain eukaryotic translation initiation factor with a range of activities.
It contains one RRM (RNA recognition) domain, a consensus RNA-binding site (RNP-CS) near the N terminus, and a central domain rich in aspartic acid, arginine, tyrosine, and glycine (DRYG).
The DRYG domain of eIF4B helps in self-association and interaction with the p170 subunit of eIF3.
eIF4B binds to both mRNA and 18S ribosomal RNA and thus acts as a bridge between the mRNA and the 40S ribosomal subunit during translation initiation.
The ATPase and RNA helicase activity of the DEAD box protein eIF4A is stimulated by eIF4B and thus functions in close association with eIF4F, a heterotrimeric protein that binds to mRNA cap structure and unwinds inhibitory secondary structure in the 5' untranslated region of eucaryotic mRNAs in an ATP-dependent manner.
Ubiquitous expression is seen in most tissues.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.005-0.1ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:K562 and RAW cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human eIF4B. Species Crossreactivity: chimpanzee, mouse, orangutan, rhesus monkey. Species sequence homology: canine, equine, rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa570-630 of human eIF4B.
