eIF3G is an RNA-associated protein involved in protein synthesis.
It is a member of the eIF-3 subunit G family and the largest of the eukaryotic translation initiation factor family with eukaryotic translation initiation factor-3 (eIF3) being a multiprotein complex composed of at least 13 different subunits.
eIF3G has a RRM (RNA recognition motif) domain and is known to interact with eIF3K.
The function of eIF3G is to bind to the 40S ribosomal subunit and promote the binding of methionyl-tRNAi and mRNA.
This subunit of eukaryotic translation initiation factor-3 also binds to the 18S rRNA.
It is ubiquitously expressed in most tissues.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 4-5ug/mlImmunohistochemistry (formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human eIF3G. Species Crossreactivity: chimpanzee, rhesus monkey. Species sequence homology: bovine, mouse, opossum, rat, xenopus, zebrafish.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa50-100 of human eIF3G.
