eIF3J is an RNA-associated protein belonging to the eukaryotic translation initiation factor family.
It belongs to the group of proteins involved in protein translation:synthesis where it binds to the 40S ribosomal subunit and promotes the binding of methionyl-tRNAi and mRNA to form the 40S initiation complex.
eIF3J is a component of the eIF3 multiprotein complex, composed of at least 13 different subunits.
It is also known to interact with eIF3S12.
Ubiquitous expression is seen in most tissues.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:293 or 3T3 cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human eIF3J. Species Crossreactivity: chimpanzee, canine, equine, mouse, orangutan. Species sequence homology: bovine, zebrafish, chicken.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Ca Eq Hu Mo
Immunogen：Synthetic peptide corresponding to aa230-280 of human eIF3J.
