EBI3 is a 228aa containing pro- and anti-inflammatory cytokine belonging to the type I cytokine receptor family and contains two fibronectin type-III domain.
It forms a heterodimer with IL-27 and is also a component of the IL-12 heterodimer.
A protein involved in regulated cell-mediated immune responses, EBI3 has an important role in regulating T helper cell development, suppressing T-cell proliferation, stimulating cytotoxic T-cell activity, inducing isotype switching in B-cells, and has diverse effects on innate immune cells.
Target cells of EBI3 include CD4 T helper cells, type 1 effector cells (TH1), type 2 effector cells (TH2) and IL-17 producing helper T-cells (TH17).
It drives rapid clonal expansion of naive but not memory CD4 T-cells.
EBI3 is important in anti-tumor as well as anti-angiogenic activity with activation and production of anti-angiogenic chemokines.
It also strongly synergizes with IL-12 to trigger interferon-gamma:IFN-gamma production of naive CD4 T-cells.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/mlImmunohistochemistry (formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes mouse EBI3. Species Crossreactivity: rat.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Mo Rt
Immunogen：Synthetic peptide corresponding to aa200-230 of mouse EBI3.
