EphA2 is a 976aa oncoprotein belonging to the ephrin receptor subfamily of the protein-tyrosine kinase family.
It consists of an external domain that includes a signal peptide, a transmembrane domain and a cytoplasmic domain that contains a canonical protein-tyrosine kinase catalytic domain.
EphA2 are the largest receptor tyrosine kinase that binds ephrin-A ligands and play an important role in axon guidance and cell migration during development of the nervous system.
Over expression of EphA2 is regulated by p53 and is known to be involved in tumor metastasis and angiogenesis.
It is highly expressed in tissues that contain a high proportion of epithelial cells, including lung, skin, small intestine, and ovary and also in metastatic melanoma cells.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/mlImmunohistochemistry (paraffin): 10ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Human ovary lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity：Recognizes human EphA2. Species Crossreactivity: bovine, canine, equine, monkey. Species sequence homology: mouse, rat, chimpanzee.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Bo Ca Eq Hu Mk Mo
Immunogen：Synthetic peptide corresponding to aa450-500 of human EphA2.
