eIF3D is a protein belonging to the largest of the eukaryotic translation initiation factor family, eukaryotic translation initiation factor-3 (eIF3).
eIF3 is a multiprotein complex composed of at least 13 different subunits.
eIF3D binds to the 40S ribosomal subunit and thus helps in maintaining the 40S and 60S ribosomal subunits in a dissociated state.
An important role of eIF3D is the formation of the 40S initiation complex by interacting with the ternary complex of eIF2:GTP:methionyl-tRNA, and promotes mRNA binding.
eIF3D is the major RNA binding subunit of the eIF3 complex.
It associates with the subunit p170 of eIF-3.
Ubiquitous expression of eIF3D is seen in most of the tissues and it is phosphorylated upon DNA damage, likely by ATM or ATR.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.005-0.1ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Brain
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human eIF3D. Species Crossreactivity: chimpanzee, macaque, mouse, orangutan, rat, rhesus monkey, zebrafish. Species sequence homology: chicken, bovine, canine, equine, opossum.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt Ze
Immunogen：Synthetic peptide corresponding to aa500-550 of human eIF3D.
