DKK1 is a 266aa secreted protein with two cysteine rich regions and belonging to the dickkopf family.
It is involved in embryonic development through its inhibition of the WNT signaling pathway and in the reentry into the cell cycle of human adult stem cells from bone marrow.
Its loss of expression plays a role in development or progression of malignant melanoma.
It decreases melanocyte function and is a key player in multiple myeloma bone disease.
It is a potential prognostic and diagnostic marker for breast cancer patients with poor prognosis.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:A549 and RAW cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity：Recognizes human DKK1. Species sequence homology: mouse, rabbit.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa150-200 of human DKK1.
