Peptidylprolyl isomerase B, also known as Cyclophilin B and PPIB, is a 216aa containing enzyme with peptidyl-prolyl cis-trans isomerase (PPIase) activity and is associated with the secretory pathways and released in biological fluids.
It belongs to the cyclophilin-type PPIase family and PPIase B subfamily with a PPIase cyclophilin-type domain and is usually located in the lumen of endoplasmic reticulum and melanosomes.
PPIases accelerate the folding of proteins and are known to catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
This cyclosporine-binding protein can also bind to cells derived from T- and B-lymphocytes, and is inhibited by the immunosuppressive drug cyclosporin A (CsA) and is hence an important element in the study of CsA-mediated immunosuppression.
Reports also suggest that PPIB is an important component of the HCV replication machinery, acting as a stimulatory regulator of NS5B and could be a possible target for antiviral therapy.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human CYPB. Species Crossreactivity: bovine, primate, canine, equine, mouse and porcine
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Bo Ca Eq Hu Mo Po
Immunogen：Synthetic peptide corresponding to aa100-228 of human CYPB. Species Sequence Homology: chicken, 84%
