G1:S-specific Cyclin-D1 protein is encoded by the CCND1 gene and it belongs to the highly conserved Cyclin Family.
Cyclin family members, including G1/S-specific Cyclin-D1, are characterized by a dramatic periodicity in protein abundance throughout the cell cycle.
Cyclin-D1 acts as an important regulator of progression from G1 to S phase.
Degradation of Cyclin-D1 results in arrest in G1 phase.
Accumulation of Cyclin-D1 is regulated at multiple levels including transcription, post-translational activation and cellular localization throughout the cell cycle.
Cyclin-D1 also acts to promote cell migration by inhibiting Rho/ROCK signaling and expression of thrombospondin-1 (TSP-1), an extracellular matrix protein that regulates cell migration in many settings including cancer.
Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis in humans.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 4-6ug/mlImmunohistochemistry (formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human CCND1. Species Crossreactivity: mouse. Species sequence homology: rat.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa250 to 300 of human CCND1.
