ACAP2 is a transport carrier belonging to the Centaurin family with three ANK repeats, an Arf-GAP domain, a BAR domain, and a PH domain.
ACAP2, a GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) has robust constitutive ARF6 GAP activity, where the GAP activity is stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidic acid but has little activity toward ARF1 and is known to control the return of ARF to the inactive GDB-bound shots.
ACAP2, along with ACAP1, is targeted by phosphatidyl inositol (3, 4, 3) triphosphate inhibiting the formation of PDGF-induced dorsal membrane ruffles in NIH3T3 fibroblasts when overexpressed.
ACAP2 is phosphorylated upon DNA damage, probably by ATM or ATR.
Expression is wide-spread with the highest level in lung.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.25-0.5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Testis
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human ACAP2. Species Crossreactivity: chimpanzee, canine, equine, monkey, mouse, rat. Species sequence homology: xenopus, chicken, opossum.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Ca Eq Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to aa350-400 of human ACAP2.
