CD28, a 44kD homodimer and T-cell membrane glycoprotein, is a member of the Ig super family.
It binds B7 molecules on APCs and transmits vital co-stimulatory signals required for proper T-cell proliferation, survival, IL-2 production and Th2 development.
CD28 plays a vital role in recruitment of signaling molecules to the TCR including PI3K, PKB/Akt, Src Kinase Lck, Tec Kinases, PTKs, Vav-1, IKKalpha, Grb2, SLP76, p21ras and PAK.
CD28/B7 interactions activate various signaling pathways including PI3K/Akt signaling, PTK-signaling, NF-kappaB activation and Rho-GTPase signaling.
CD28 co-stimulation and ligation with TCR/CD3 complex directly controls T-cell cycle progression by down-regulating CDK inhibitor p27Kip1.
These interactions also up-regulate Bcl-XL expression and down-regulate FasL expression thus playing a vital role in T-cell survival.
Blocking of CD28/B7 pathway results in immunosuppression with implications for treatment of autoimmune diseases, organ transplantation and graft vs.
host disease.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human CD28.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa72-85 [SQQLQVYSKTGFN].
