CNOT4 is a 575aa protein with E3 ubiquitin ligase activity, mainly involved in protein modification and protein ubiquitination.
It contains a ring type zinc finger domain of the C4C4 type, an RNA recognition motif and a bipartite nuclear localization signal.
Studies show that CNOT4 is a component of the CCR4-NOT (CAF1) core complex that functions as general transcription regulation complex and has both a positive as well as a negative effect on the transcription of multiple functionally unrelated genes, thus controls gene expression.
CNOT4 interacts with CNOT1 via C-terminus and with E2 ubiquitin ligases via C4C4 RING domain.
Reports suggest that CNOT4 gets phosphorylated in response to DNA damage on consensus sites recognized by ATM and ATR.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.05-0.1ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Human skeletal muscle lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human CNOT4. Species Crossreactivity: chicken, bovine, mouse, rat, xenopus. Species sequence homology: pufferfish.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Bo Ch Hu Mo Rt Xe
Immunogen：Synthetic peptide corresponding to aa50-100 of human CNOT4.
