84-1191-42　Caspase-12 (CASP-12, Casp12) 100ug　C2088-59C

特徴

• Caspases are cysteine proteases that cleave C-terminal aspartic acid residues on their substrate molecules.
• Caspase-12 (CASP12) process inflammatory cytokines.
• It attenuates the macrophage-elicited T helper cell type 1 and type 2 cytokine response, with probable compensatory enabling of T cell-derived interferon-gamma formation.
• Casp12Dmpened the production of the proinflammatory cytokines interleukin 1-beta, IL-18 and IFNg, but not tumor necrosis factor-alpha and IL-6, in response to various bacterial components that stimulate Toll-like receptor and NOD pathways.
• In rodents, Casp12 mediates apoptosis in response to endoplasmic reticulum stress.
• However, in human, CASP12 appears to be nonfunctional.
• A SNP in CASP12 in humans results in the synthesis of either a truncated protein (Csp12S) or a full-length caspase proenzyme (Csp12L).
• SNP encoding Csp12L is confined to populations of African descent and confers hyporesponsiveness to lipopolysaccharide-stimulated cytokine production in ex vivo whole blood but has no significant effect on apoptotic sensitivity.
• PCR analysis detected highest expression of CASP12 in lung.
• Expression was intermediate in small intestine, stomach, and kidney.
• Nine CASP12 splice variants are identified in lung and small intestine.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.25-1ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control:Skeletal muscle
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.