Apoptosis, or programmed cell death, is a common property of all multicellular organisms.
The current dogma of apoptosis suggests that the components of the core cell-death machinery are integral to cells and widely conserved across species.
Caspases, a family of cysteinyl aspartate-specific proteases, are integral components of the cell death machinery (reviewed in Siegal, 2006; and Lavrik et al, 2005).
They play a central role in the initiation and execution of apoptotic cell death and in inflammation.
Caspases are typically divided into 3 major groups, depending on the structure of their prodomain and their function.
Group II: initiator of apoptosis caspases (caspases 2, 8, 9).
Group II: effector caspases (caspases 3, 6, 7).
Caspases are synthesized as zymogens (inactive pro enzyme precursors which require a biochemical change to become active enzymes) with an N-terminal prodomain of variable length followed by a large subunit (p20) and a small subunit (p10).
Caspases are activated through proteolytic cleavage at specific asparagine residues that are located within the prodomain, the p10, and p20 subunits.
Activation results in the generation of mature active caspases that consist of the heterotetramer p202-p102.
Active caspases mediate cell death and inflammation through cleavage of particular cellular substrates that are involved in these processes.
Applications:Suitable for use in Western Blot, Immunohistochemistry (frozen, paraffin) and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:2000Immunohistochemistry (formalin fixed paraffin embedded): 1:1000-1:5000Immunoprecipitation: 1:50-1:200Immunohistochemistry: FrozenOptimal dilutions to be determined by the researcher.
Positive Control:CNS lymphatic tissues, CA cell lines
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.05% sodium azide.
Specificity：Recognizes the proform of human caspase-9 (~50kD), and the large (~35kD) subunit of active/cleaved Caspase-9. It does not recognize the small (~15kD) subunit of active/cleaved Caspase-9. Intermediate caspase-9 cleavage forms may also be seen at ~21kD.
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa188-206 (EKLRRRFSSLHFMVEVKGD) of human caspase-9; GenBank no. gi|27802691|gb|AAO21133.1|.
