Apoptosis, or programmed cell death, is a common property of all multicellular organisms.
The current dogma of apoptosis suggests that the components of the core cell-death machinery are integral to cells and widely conserved across species.
Caspases, a family of cysteinyl aspartate-specific proteases, are integral components of the cell death machinery (reviewed in Siegal, 2006; and Lavrik et al, 2005).
They play a central role in the initiation and execution of apoptotic cell death and in inflammation.
Caspases are typically divided into 3 major groups, depending on the structure of their prodomain and their function.
Group II: initiator of apoptosis caspases (caspases 2, 8, 9).
Group II: effector caspases (caspases 3, 6, 7).
Caspases are synthesized as zymogens (inactive pro enzyme precursors which require a biochemical change to become active enzymes) with an N-terminal prodomain of variable length followed by a large subunit (p20) and a small subunit (p10).
Caspases are activated through proteolytic cleavage at specific asparagine residues that are located within the prodomain, the p10, and p20 subunits.
Activation results in the generation of mature active caspases that consist of the heterotetramer p202-p102.
Active caspases mediate cell death and inflammation through cleavage of particular cellular substrates that are involved in these processes.
Applications:Suitable for use in Western Blot, Immunohistochemistry, ChIP and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000-1:2000Immunohistochemistry (FFPE): 1:1000-1:5000Immunoprecipitation: 1:50-1:200ChIP: 1:10-1:500Immunohistochemistry: FrozenOptimal dilutions to be determined by the researcher.
Positive Controls:Jurkat, most cancer cell lines, lymphoid tissue.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.05% sodium azide.
Specificity：Recognizes the proform of human caspase-3 (~32kD), and the large (~14-21kD) and small (~10kD) subunits of active/cleaved Caspase-3. Species Crossreactivity: canine, gerbil, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：ChIP IHC IP WB
Calc Crossreactivity：Ca Hu Mo Rt
Immunogen：Recombinant protein corresponding to full-length human Caspase-3 (pro-form).
