Beta-catenin, an adherens junction protein, was originally identified as a component of cell-cell adhesion structures.
It interacts with the cytoplasmic domain of E-cadherin and links E-cadherin to alpha-catenin, which in turn mediates anchorage of the E-cadherin complex to the cortical actin cytoskeleton.
Recent results show that it also binds to another cytoskeletal complex containing the adenomatous polyposis coli protein and microtubules, and interacts with several signaling pathways that include tyrosine kinases and phosphatases and Wnt/Wingless.
Interplay between these cytoskeletal complexes and signaling pathways may regulate morphogenesis.
The protein is expressed in several hair follicle cell types, basal and peripheral matrix cells, and cells of the outer and inner root sheats.
A pathological role of b-catenin has been identified in pilomatrixoma, medulloblastoma and tumor development.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.25-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：20ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human beta catenin, phosphorylated at Ser33/Ser37. Species Crossreactivity: chicken, chimpanzee, mouse, opossum, orangutan, rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Ch Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human Beta-catenin protein containing phosphorylated serine residues at position 33 and 37.
