BCLAF1 is an intracellular, death promoting transcriptional repressor of survival genes that interacts with several members of the BCL2 family of proteins.
It shows homology to the basic leucine zipper (bzip) and MYB DNA binding domains.
From recent research it can be concluded that in case of apoptotic cells this protein localizes to dot-like structures throughout the nucleus, and redistributes to a zone near the nuclear envelope.
When over expressed this protein induces apoptosis and can be suppressed by co-expression of BCL2 proteins.
Also known as BTF, it triggers a proapoptotic signal, potentially helpful to overcome the resistance to STI571.
BCLAF1 is found to express mostly in skeletal muscles.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/mlImmunohistochemistry (paraffin): 10ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Jurkat cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity：Recognizes human BCLAF1. Species Crossreactivity: chimpanzee, canine, mouse. Species sequence homology: mosquito, chicken.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Ca Hu Mo
Immunogen：Synthetic peptide corresponding to aa700-750 of human BCLAF1.
