Brachyury is a 435aa containing embryonic nuclear transcription factor binding to the palindromic T-site.
It binds to the T-box in the N-terminus to regulate the genes required for mesodermal differentiation.
It is the earliest indicator of mesoderm formation and differentiation.
Brachyury is expressed during gastrula and neurula stages in involuting mesoderm and in the notochord.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1-3ug/mlImmunohistochemistry (formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human Brachyury. Species Crossreactivity: chimpanzee, rhesus monkey. Species sequence homology: mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mk
Immunogen：Synthetic peptide corresponding to aa350-400 of human Brachyury
