84-1191-23　BMI-1 (Polycomb Complex Protein BMI-1, Polycomb Group RING Finger Protein 4, RING Finger Protein 51, BMI1, PCGF4, RNF51) 100ug　B2185-02D

特徴

• BMI1 is a 326aa containing protooncogene belonging to the polycomb gene family with a RING-type zinc finger domain.
• A transcription factor and a senescence regulator, BMI1 is a component of the Polycomb group (PcG) multiprotein PRC1 complex, required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development.
• An important role of BMI1 has been reported in regulating proliferation of stem cells and tumor cells in diverse adult epithelial tissues involved in human colorectal carcinogenesis by repressing the INK4a:ARF proteins (CDKN2A).
• This protein also plays an essential role in the generation of self-renewing adult hematopoietic stem cells and in the maintenance and expansion of immature granule cell precursors.
• BMI1 cooperates with the MYC oncogene to produce B-lymphomas.
• Ubiquitous expression is seen in most of the tissues.
• Applications:Suitable for use in Western Blot, Immunocytochemistry, Immunofluorescence and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 2-3ug/mlImmunohistochemistry (Paraffin): 5ug/mlImmunofluorescence (IC): 5ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.