PRDM1, an 88kD novel transcription repressor protein is a human homolog of mouse Blimp1.
It belongs to the family of PRDM protein family and contains a PR domain and 5 Kruppel Like-zinc finger motifs at the C-terminal region.
It plays a pivotal role in inducing and regulating terminal differentiation of B-lymphocytes.
It recruits HDAC and complex of Groucho/TLE family proteins to the DNA and actively represses vital genes required for B-cell proliferation including c-myc, CIITA promoter III, CD23b and PAX-5 promoter.
PRDM1 silences IFNâ-gene expression by binding specifically to PRDI of the IFNâ gene promoter.
It is continuously expressed in all Plasma cells, but is not detected in early bone marrow B-cells, memory B-cells in spleen and most germinal center B-cells.
In myeloma cells, PRDI-BF1â, a truncated isoform of PRDM1 is expressed that has an impaired ability to repress transcription suggesting a clinical implication.
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum.
Form：Supplied as a liquid, 0.025% sodium azide, 50% glycerol.
Specificity：Recognizes human BLIMP-1. Species sequence homology: bovine, chicken, chimpanzee, canine, mouse, rat, zebrafish.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa692-709 of human PRDM1.
