84-1191-20　BIRC2 (Baculoviral IAP Repeat-containing Protein 2, C-IAP1, IAP Homolog B, Inhibitor of Apoptosis Protein 2, IAP-2, hIAP-2, hIAP2, RING Finger Protein 48, TNFR2-TRAF-signaling Complex Protein 2, API1, IAP2, MIHB, RNF48) 50ul　B2049-90D

特徴

• cIAP1 (HIAP2, MIHB) is a member of the family of inhibitor of apoptosis proteins (IAP).
• IAPs suppress mitochondria- dependent and -independent apoptosis by binding to and inhibiting caspases through their BIR domains.
• Resistance towards apoptosis is a hallmark of cancer cells, and overexpression of IAPs can contribute to the development of cancer though inhibiting apoptosis.
• In addition to at least one BIR domain, some IAP members also have a RING-type finger motif at their carboxyl-terminal.
• The RING finger domain of several IAPs, including cIAP2, have E3 ubiquitin ligase activity and target the degradation of Smac/DIABLO through ubqiuitination.
• Smac/DIABLO is a death inducer and functions by inhibiting IAP-caspase interactions, thereby promoting apoptosis.
• Degradation of cell death inducers like Smac/DIABLO is thought to be a conserved mechanism by which IAPs enhance their anti-apoptotic activity, thereby promoting cell survival.
• The IAPs, including cIAP1, have widespread tissue protein expression, with expression levels and subcellular localization patterns differing depending on the cell lineage.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:1000-1:2000Immunohistochemistry (Paraffin): 1:1000-1:5000Immunohistochemistry: Frozen sectionsImmunoprecipitation: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Positive Control:Epidermis CNS lymphatic tissues, many cancer cell lines
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.