84-1191-12　Bcl 2 (B Cell Leukemia 2, B Cell Leukemia/lymphoma 2, B Cell Lymphoma 2, B Cell CLL/lymphoma 2, Bcl2, Bcl-2 Protein, Apoptosis Regulator Bcl 2, Apoptosis Regulator Bcl2) 50ul　B0807-03Q

特徴

• The Bcl-2 family of apoptosis-related genes plays central roles in regulating apoptotic pathways (reviewed in Thomadaki and Scorilas, 2006).
• Regulation of cell death through apoptosis is critical for the maintenance of homeostasis, defense against infectious agents, and normal development.
• Bcl-2 family proteins regulate apoptosis primarily through the regulation of mitochondrial outer membrane permeability.
• In mammals, the family consists of both prosurvival (antiapoptotic) and proapoptotic (prodeath) members.
• Cellular homeostasis is thought to be dependent on a balance between the actions of prosurvival and proapoptotic proteins.
• Bcl-2 family proteins can be divided into 3 main subfamilies on the basis of their function and the content of their Bcl-2 homology (BH) domains, for example: 1) Prosurvival: Bcl-2, Bcl-XL, Bcl-W, A1, and Mcl-1 2) Proapoptotic (multidomain): Bax, Bak, and Bok.
• 3) BH3-only (proapoptotic): Bad, Bcl-XS, Bid, Bik, Bim, Blk, Bmf, Bnip, Noxa, and Puma.
• Prosurvival members inhibit cells from undergoing apoptosis, whereas proapoptotic and BH3-only subfamily members promote apoptosis.
• There are 4 BH domains (1-4) conserved among Bcl-2 family proteins.
• The BH domains are important for function as well as for heterodimerization between family members.
• Typical prosurvival family members have all four BH domains (1-4), whereas proapoptotic (multidomain) members have BH1, 2 and 3 domains and BH3-only members have only the BH3 domain.
• Overall, the relative ratio of prosurvival and proapoptotic proteins determines the suseptibility of a cell to various apoptotic stimuli.
• Many Bcl-2 family proteins are differentially expressed in various malignancies and some are useful prognostic biomarkers.
• Prosurvival proteins are often elevated in diverse cancers and have the potential to confer resistance to both endogenous cell death stimuli and cancer treatments.
• Alterations in the ratio or levels of Bcl-2 family proteins have been also associated with nonmalignant diseases including neurodegenerative diseases, autoimmune diseases, AIDs, Down’s syndrome, cardiovascular diseases, diabetes, glomerulonephritis, and muscular dystrophy.
• Recognizes both Bcl-2 alpha and beta isoforms.
• Human Bcl-2 alpha is a 239aa protein and human Bcl-2 beta is a 205aa protein.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:2000Immunohistochemistry (FFPE): 1:1000-1:5000 Requires HIER using sodium citrate buffer pH6.0 prior to staining
• Immunoprecipitation: 1:50-1:200Immunohistochemistry: FrozenOptimal dilutions to be determined by the researcher
• Positive Control:Lymphoid, CA cell lines
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.