AMP deaminase (AMPD) is an allosteric enzyme involved in the regulation of adenosine metabolism.
It catalyzes a central reaction in purine nucleotide biosynthesis where AMP is deaminated to IMP liberating ammonia.
There are three functional isoforms of AMPD.
AMPD1 (E.C.
No 3.5.4.6.) is the skeletal muscle-specific isoform M located in type II muscle fibers, neuromuscular junctions and in capillaries.
This protein is the predominant member of AMPD multi-gene family and is considered as the sensor of the cell's changing energy requirements.
AMPD1 deficiency causes irregular muscle metabolism due to lower rate of ATP degradation, phosphocreatine hydrolysis and accumulation of lactic acid.
Mutated AMPD1 expression has been detected in neuromuscular disorders, exercise-induced skeletal muscle myopathies and congestive heart failures due to coronary artery diseases.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/mlImmunohistochemistry (paraffin): 10ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Testis
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity：Recognizes human AMPD1. Species Crossreactivity: bovine, canine, monkey, mouse, ratSpecies sequence homology: chimpanzee, zebrafish.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Bo Ca Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa140-190 of human AMPD1.
