Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway, is important for normal growth control and may be defective in tumor cells.
A member of the autophagy family of proteins is APG7L which was identified in yeast as a ubiquitin-E1-like enzyme; this function is conserved in the mammalian homolog.
In mammalian cells, APG7L is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles.
The protein thus plays an indispensable role in the initial step of the conjugation system.
APG7L interacts with Apg8p/Aut7p and Aut1p:Apg3p in addition to Apg12p.
The protein maps to 3p25.3-p25.2 region of human chromosome.
Ablation of the protein leads to abnormal swellings and dystrophy of purkinje cell axon terminals in the deep cerebellar nuclei (DCN).
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1-3ug/mlImmunohistochemistry (formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human APG7L.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa550-600 of human APG7L.
