84-1190-88　ASK1 (Apoptosis Signal-regulating Kinase 1, ASK-1, Mitogen-activated Protein Kinase Kinase Kinase 5, MAP3K5, MAPKKK5, MAPK/ERK Kinase Kinase 5, MEK Kinase 5, MEKK 5, MEKK5) 50ul　A3794-05B

特徴

• Ask1 (apoptosis signal-regulating kinase 1), a serine/threonine kinase, is a member of the MAPKKK family that constitutes the JNK and p38 (MAPK) cascades.
• Members of this family are activated by physiological and cytotoxic stresses and induce various stress responses including apoptosis (reviewed in Sekine et al.
• 2006).
• Specifically, Ask1-MAPK cascades have been shown to be involved in endoplasmic reticululum (ER) stress-induced apoptosis and in neuronal cell death.
• Studies suggest that ER stress-induced apoptosis plays critical roles in the pathogenesis in some neurodegenerative diseases including Alzheimer's, Parkinson's, polyglutamine (polyQ), amyotrophic lateral sclerosis (ALS) and Prion diseases.
• Studies using using cultured mammalian cells suggest that Ask1 may play a role as a proapoptotic signaling intermediate in the pathogenesis of these and other neurodegenerative diseases that involve ER stress-induced apoptosis.
• However, the regulation and function of Ask1 in neuronal cell death remains to be fully elucidated.
• In addition to playing a role in ER stress-induced apoptosis, Ask1 is also thought to be important in cytokine-induced apoptosis, and may also have functions other than apoptosis including roles in cell survival and differentiation.
• Structurally, human Ask1 has a serine/threonine kinase domain in the middle of the molecule.
• The kinase domain is evolutionarily conserved from nematode to human.
• A threonine residue with the activation loop of the kinases domain (Thr838 of human Ask1) is essential for Ask1 activation.
• There are two coiled-coil domains, one in the N-terminal and the other in the C-terminal domain.
• The coiled-coil domain in the C-terminal domain has been shown to be required for homo-oligomerzation.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:2000Immunohistochemistry (formalin fixed paraffin embedded): 1:1000-1:5000Immunoprecipitation: 1:50-1:200Immunohistochemistry: FrozenOptimal dilutions to be determined by the researcher.
• Positive Control:Spleen, lymphoid, many cancer cell lines
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.