84-1190-81　ASAP2 (Arf-GAP with SH3 Domain, ANK Repeat and PH Domain-containing Protein 2, Development and Differentiation-enhancing Factor 2, Paxillin-associated Protein with ARF GAP Activity 3, PAG3, Pyk2 C-terminus-associated Protein, PAP, DDEF2, KIAA0400) 100ug　A3334-96

特徴

• ASAP2 is a multidomain protein belonging to the beta family with two ANK repeats, an Arf-GAP domain, a PH domain and a SH3 domain.
• This protein localizes in the Golgi apparatus and also at the plasma membrane where it colocalizes with protein tyrosine kinase 2-beta (PYK2) and forms a stable complex with PYK2 in vivo.
• ASAP2 is tyrosine phosphorylated by activated PYK2.
• It functions as a substrate and downstream target for PYK2 and SRC, a pathway that may be involved in the regulation of vesicular transport.
• ASAP2 activates the small GTPases ARF1, ARF5 and ARF6 and regulates the formation of post-Golgi vesicles and modulates constitutive secretion.
• It modulates phagocytosis mediated by Fc gamma receptor and ARF6.
• Usually detected in heart, brain, placenta, kidney, monocytes and pancreas, it is up-regulated during monocyte maturation.
• Multiple transcript variants encoding different isoforms have been found.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-2ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control:Human Brain lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.