Human apurinic (apyrimidinic) endonuclease:redox-factor 1 (APE/Ref-1) is involved in the repair of DNA damage as well as in the transcriptional regulation of genes.
The repair function of the protein is located on its C-terminal region.
Activation of transcription factors, which occurs via a redox-based mechanism, pertains to a approximately 6kD N-terminal fragment with Cys-65 being of essential importance.
Ape1/ref-1 has been shown to stimulate the DNA binding activity of numerous transcription factors that are involved in cancer promotion and progression such as Fos, Jun, NFkB, PAX, HIF-1, HLF and p53.
APE/Ref-1 is part of the cellular response to oxidative stress and protects cells from the genotoxic and cytotoxic effect of oxidizing agents.
Thus, specific downmodulation of APE:Ref-1 activity in tumors might be considered as a novel therapeutic approach in tumor therapy.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:HeLa
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human APE-1.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptides corresponding to aa36-52 and 216-232 of human APE (KLH).
