84-1190-72　AIFM1 (Programmed Cell Death Protein 8, Apoptosis-inducing Factor 1, Mitochondrial, AIF, PDCD8) 50ul　A1372-32C

特徴

• AIF (apoptosis-inducing factor) was initially discovered as a protein involved in caspase-independent cell death.
• It is now known that AIF has both vital and lethal functions (reviewed in Modjtahedi et al, 2006).
• In healthy cells, AIF is a flavoprotein present in the mitochondria where it has vital roles in cellular redox metabolism and mitochondrial bioenergetics.
• In many models of apoptosis, AIF is released from the mitochondria during mitochondrial outer membrane permeabilization along with other mitochondrial proteins.
• Upon release, AIF translocates first to the cytosol and then to the nucleus where it induces chromatin condensation and DNA degradation.
• Although several lines of evidence suggest that AIF is a main mediator of capsase-independent cell death, the mechanisms regulating AIF pro-apoptotic function remain to be fully elucidatied and may depend on the cell type and type of apoptotic stimuli.
• Human AIF is transcribed from a nuclear gene located on the X chromosome and translated in the cytoplasm to a precursor protein of 613aa which corresponds to ~67kD.
• The precursor protein is imported into the mitochonria by mitochondrial localization sequences located within the N-terminal prodomain of AIF.
• Once inside the mitochondria, the prodomain is cleaved giving rise to a mature AIF form of ~57kD.
• AIF isoforms generated from a single AIF gene have been identified, including AIF short isoform 2 (324aa protein; GenBank no.
• gbAAY84739.1) and AIF short isoform 3 (237aa protein; GenBank no.
• gbAAY84741.1) (reviewed in Delttre et al, 2006).
• The generation of multiple isoforms from a common gene is an evolutionary mechanism that increases protein diversiity in eukaryotes.
• Regulating gene expression through the production of multiple isoforms from a single gene is thought to play a major role in the control of apoptosis and other forms of programmed cell death.
• Recognizes AIF and AIF isoforms containing the peptide immunogen sequence, RARDPGARVLIVSEDPELP.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:2000Immunohistochemistry (formalin fixed paraffin embedded): 1:1000-1:5000Immunoprecipitation: 1:50-1:200Immunohistochemistry: FrozenOptimal dilutions to be determined by the researcher.
• Positive Control:Spleen, lymphoid, many cancer cell lines
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.