AKT, protein kinase B (PKB), is a serine/threonine kinase which is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activat phosphtidylinositol 3-kinase (PI 3-kinase).
The major activity of AKT/PKB is to mediate cell survival.
AKT/PKB is also believed to be a critical factor in the genesis of cancer as the tumor suppressor PTEN was found to antagonise PI-3 kinase and AKT/PKB kinase activity.
AKT/PKB phosphorylation is critical for it activity.
The major phosphorylation sites required for AKT activation has been identified as threoinine 308 and serine 473.
Serine 473 is phosphorylated by MAPKAP kinase 2.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Positive Control:Jurkat
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, 0.1mg/ml BSA, 0.02% sodium azide.
Specificity：Recognizes human AKT1. Species sequence homology: chicken, frog, mouse.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa464-477 of human AKT1.
