Agrin is an essential extracellular matrix component which promotes clustering of nicotinic acetylcholine receptors (nAChRs) and other proteins during development at the neuromuscular junction.
Agrin, MuSK and Rapsyn are all essential components for AChR aggregation, through an unknown mechanism.
The C-terminal region of agrin is released into the medium, interacts with receptors on the muscle surface and induces AChR aggregation.
The central region contains two O-linked glycosylation sites and a domain homologous to domain III of laminin.
The N-terminal region anchors agrin to the extracellular matrix via other basal membrane components.
This region also contains a protease inhibitor domain and glycosaminoglycan attachment sites; increasing the predicted MW from 200kD to ~600kD.
Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:100Immunohistochemistry (formalin fixed paraffin embedded): 1:100Immunohistochemistry: FrozenOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS. No preservative added.
Specificity：Recognizes human AGR2. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptides corresponding to aa55-72 (TQTYEEALYKSKTSNKPL) of human AGR2 (NP_006399.1). Species sequence homology: bovine, chicken, chimpanzee, canine, orangutan, rat, xenopus.
