AGAP2 (also known as PIKE-A), a putative oncogene, shows elevated expression in cancer cells compared to non-transformed cells and has been implicated as regulators of cancer cell invasion into normal tissues.
A contributor to brain, eye, breast and liver cancers, AGAP2 belongs to the centaurin gamma-like family with two ANK repeats, an Arf-GAP domain, a Miro domain and a PH domain.
It is a GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity.
The Arf-GAP domain interacts with G domain and may regulate its GTPase activity.
GAP activity of AGAP2 is stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and, to a lesser extent, by phosphatidylinositol 3,4,5-trisphosphate (PIP3).
Phosphatidic acid potentiates PIP2 stimulation.
Several isoforms of AGAP2 have been reported.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 2-3ug/mlImmunohistochemistry (formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human AGAP2. Species Crossreactivity: monkey. Species sequence homology: equine.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mk
Immunogen：Synthetic peptide corresponding to aa20-60 of human AGAP2.
