ZAP70 (Zeta-associated protein 70kD also Syk-related tyrosine kinase) is a 68-70kD member of the SYK subfamily, tyrosine kinase family of molecules.
It is found in immature thymocytes, plus T cells and NK cells, and is essential for TCR-mediated IL-2 production, plus the development of CD8+ T cells.
Human ZAP70 is 619aa in length.
It contains two SH2 domains (aa10-102) and (aa162-254), a regulatory interdomain region (aa255-337), and a C-terminal tyrosine kinase domain (aa338-539).
The two SH2 domains bind to phosphorylated tyrosines on the CD3 z-chain following TCR activation.
This activates the ZAP70 kinase domain with the initiation of downstream signaling via SLP76 and LAT.
There are four potential splice variants of ZAP70.
One termed TZK is 37kD in size, and characterized by an alternative start site at Met308.
This is expressed in very early thymocytes.
Two others show an alternative start site 88aa upstream of the standard site, with one of these variants also showing a deletion of (aa495-541).
A fourth variant contains an eight aa substitution for (aa1-134).
Over (aa10-102), human ZAP70 shares 98% aa identity with mouse ZAP70.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS with 5% trehalose. Reconstitute with sterile PBS.
Specificity：Recognizes human ZAP70.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to aa10-102 from human ZAP70 expressed in E. coli (P43403).
Shelf Life：1year
Swiss Prot Number：P43403
EU Commodity Code：30021010
商品名：ZAP 70, (Zeta Chain Associated Protein Kinase 70kD, Zeta Chain Associated Protein Kinase 70kD, Zeta-chain Associated Protein Kinase 70kD Isoform 1, Zeta-chain Associated Protein Kinase 70kD Isoform 2, Zeta Chain TCR Associated Protein Kinase 70kD, Zeta-chain (TCR) Associated Protein Kinase 70kD, ZAP70, ZAP-70, 70kD Zeta Associated Protein, EC 2.7.10.2, FLJ17670, FLJ17679, Selective T Cell Defect, STD, Syk-related Tyrosine Kinase, SRK, Truncated ZAP Kinase, TZK, Tyrosine Protein Kinase ZAP70) 100ug
