XPB (Xeroderma pigmentosum group B complementing protein; also ERCC3 and TFIIH 89kD subunit) is an 86kD, nuclear member of the RAD25/XPB subfamily, helicase family of proteins.
It is expressed in multiple cell types, including neurons, fibroblasts and keratinocytes.
XPB is part of the 10 subunit TFIIH complex.
As such, it appears to participate in both nucleotide excision repair (NER) and transcription initiation from RNA polymerase II promoters.
During NER, XPB utilizes an intrinsic ATPase activity to resolve nucleotide excision and repair.
Human XPB is 782aa in length.
It contains an NLS (aa6-18), multiple acidic-rich regions, an ATPase region (aa 327-488) and one helicase domain (aa542-702).
Over (aa1-375), human XPB shares 93% aa identity with mouse XPB.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS.
Specificity：Recognizes human XPB. Species crossreactivity: mouse, rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant corresponding to aa1-375 from human XPB expressed in E. coli (P19447).
