84-1190-36　TopBP1 (DNA Topoisomerase 2 Binding Protein 1, DNA Topoisomerase II Binding Protein, DNA Topoisomerase II Binding Protein 1, DNA Topoisomerase IIbeta Binding Protein 1, Hypothetical Protein KIAA0259 [Fragment], KIAA0259, Topoisomerase (DNA) II Binding Protein 1, Topoisomerase II Binding Protein 1, TOP2BP1) 100ug　T8064-59

特徴

• TOP2B (DNA Topoisomerase II b) is a 180-185kD member of the type IIA subfamily, topoisomerase family of molecules.
• It is ubiquitously expressed, and represents the larger of two known topoisomerases (the 2A/a-form being 170kD in size).
• TOP2A is essential for cell division, while TOP2B is active postmitotically.
• In an ATP-dependent manner, homodimeric TOP2B relieves torsional stress created in DNA during transcription, or as a consequence of replication.
• TOP2B first induces cleavage of double-stranded DNA, creating a space that allows for the physical repositioning of chromatin and a reduction in tension.
• This is followed by closure and ligation of the cleaved ends to recreate the original DNA structure.
• Human TOP2B is 1626aa in length.
• It contains an ATPase domain (aa101-201), an NES (aa1034-1044), more that 30 Ser/Thr phosphorylation sites and a C-terminal DTHCT region (aa1508-1611).
• There is one splice variant that shows a deletion of (aa24-28).
• Over (aa1187-1621), human TOP2B shares 91% aa identity with mouse TOP2B.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.2ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.