84-1190-35　Thymosin B10 100ug　T5225-08P

特徴

• Thymosin beta 10 (TB10) is a 6.5kD member of the Bthymosin family of molecules.
• Members of this family range from 41-44aa in length, and possess an isoelectric point that lies between pH 5.07.0.
• Multiple cell types produce TB10, either constitutively, or after stimulation.
• They include monocytes/macrophages, endothelial cells, thyroid epithelium, spermatids, fetal cerebellar Purkinje cells plus renal tubule epithelium, and multiple tumor types.
• TB10 isprincipally an intracellular molecule.
• It forms a 1:1 complex with Gactin and blocks Factinpolymerization.
• This regulates the availablility of actin monomers for filament formation and subsequent cell migration.
• It is also known to be noncanonically secreted, acting on endothelium and blocking angiogenesis.
• Mature human TB10 is 43aa in length (aa244) It contains an actinbinding site (aa1823), an Nterminal acetylated alanine, four acetylated lysines (#4;15;26; 39), and one phosphorylation site at Thr23.
• Mature TB10 is identical to mouse TB10 in aa sequence, and it shares 74% aa sequence identity with its human family member TB4.
• Human Thymosin B10.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1mg/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.