TDRKH (Tudor domain and KH domain containing protein; also TDRD2) is a 70kD member of the Tudor family of proteins.
It is expressed in developing spermatogonia and meiotic primary spermatocytes, but not embryonic stem cells.
TDRKH forms part of an RNA-silencing complex that safeguards the germ cell genome.
Through its 60aa Tudor domain, it binds methylated PIWI (P-element-induced wimpy testis) protein and serves to position PIWI for non-coding RNA binding.
Human TDRKH is 606aa in length.
It contains two RNA recognition KH domains (aa52-115 )and (124-190), and one methyl-binding Tudor domain (aa353-412).
There are four potential splice forms.
Three possess a shared deletion of (aa562-606), with two of these also showing a unique deletion of (aa108-152) and 76-79, respectively; a fourth shows a two aa substitution for (aa559-606).
Over (aa206-561), human TDRKH shares 87% aa identity with mouse TDRKH.
Applications:Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 5-15ug/mlWestern Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS with 5% trehalose. Reconstitute with sterile PBS.
Specificity：Recognizes human TDRKH.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to His206Leu561 form human TDRKH expressed in E. coli (Q9Y2W6).
