Teneurin-1(also Ten-m1, tenascin-M1, and Ten-m/Odz1) is a 250-300kD member of the tenascin family, teneurin subfamily of transmembrane (TM) molecules.
It is a covalently-linked homodimer that is expressed in both embryonic and adult neurons, among which are cerebellar granule cells and CA2 pyramidal hippocampal neurons.
Teneurin-1 appears to promote neurite outgrowth and mediate cell-to-cell adhesion via homophilic interactions.
Human teneurin-1 is a 2725aa type II TM glycoprotein.
It contains a 324aa cytoplasmic region (aa1-324) that contains an NLS (aa62-65), plus a 2380aa extracellular domain (ECD).
The ECD possesses eight sequential EGF-like domains (aa528-796), five NHL repeats, each of which form a b-propeller (aa1194-1524), and 23 YD/TyrAsp-containing repeats that bind carbohydrates.
Cleavage at the N-terminus generates an initial 65kD membrane-spanning fragment, followed by TM cleavage that generates a 45kD cytosolic fragment.
C-terminal cleavage generates a short 5kD 41aa peptide (aa2682-2722) termed TCAP-1 that shows bioactivity.
One splice variant shows a deletion of (aa1232-1239).
Over (aa1-317, human Teneurin-1 shares 96% aa identity with mouse Teneurin-1.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Direct ELISA: 0.5-1ug/mlWestern Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS with 5% trehalose. Reconstitute with sterile PBS.
Specificity：Recognizes human Teneurin-1.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to human Teneurin-1 expressed in E. coli (AAFO4723).
