SorLA (Sorting protein-related receptor containing LDLR class A repeats; also LR11 and SorL1) is a 250-330kD, monomeric member of the SORL1 family of proteins.
It is expressed on the cell surface (10%) and in Golgi/ER membranes (90%) of neurons and vascular smooth muscle cells.
SorLA binds apoE-rich lipoproteins plus the 39kD RAP.
It also acts as an intracellular sorting receptor for APP.
In this context, it blocks transport of APP into compartments that exhibit b-secretase activity, thus interfering with Ab peptide generation.
Human SorLA is a type I transmembrane glycoprotein 2214aa in length.
It contains a 28aa signal sequence, a 53aa propeptide, and a 2133aa mature molecule.
The mature molecule contains a 2056aa extracellular domain (ECD) (aa82-2137) and a short 56aa cytoplasmic tail.
The ECD is highly modular, containing five BNR/Asp-box repeats (aa136-573), five LDLR class B repeats (aa 800-1013), one EGF-like domain (aa1026-1072), 11 LDLR class A domains (aa1076-1551) and six FN type III repeats (aa1554-2023).
Multiple potential splice variants exist.
There are alternative start sites at Met1057 and Met1155, plus a 63aa substitution for (aa1-1153) and a 19aa substitution for (aa1-1404).
Over (aa82-367, human SorLA shares 93% aa identity with mouse SorLA.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Direct ELISA: 0.5-1ug/mlWestern Blot: 1ug/mlImmunohistochemistry: 3-10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS with 5% trehalose. Reconstitute with sterile PBS.
Specificity：Recognizes human and mouse SorLA.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to human SorLA expressed in E. coli (Q92673).
