Serpin A9 (Serine proteinase inhibitor-clade A #9; also GCET1, Centerin and Serpin A11) is a 46 kD, secreted member of the a1-antitrypsin (clade A)-subfamily, serpin superfamily of protease inhibitors.
It is only produced by normal and lymphoma germinal center B cells.
Its function is unknown, but it presumably acts as an inhibitor of a trypsin-like protease.
Mature human Serpin A9 is presumably 394aa in length.
It contains one Serpin motif (aa405-415).
There are multiple splice variants associated with Serpin A9.
There are alternate start sites at Met19, Met99 and Met150.
A fourth isoform shows a two aa substitution for (aa369-435), while a fifth isoform shares this same substitution coupled with a 39aa substitution for (aa1-75).
A sixth variant shows a deletion of (aa91-190).
Mature human Serpin A9 shares 67% aa identity with mouse Serpin A9.
Applications:Suitable for use in ELISA, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Direct ELISA: 0.5-1.0ug/mlImmunohistochemistry: 3-10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS with 5% trehalose. Reconstitute with sterile PBS.
Specificity：Recognizes human Serpin A9.
Isotype：IgG
Calc Applications Abbrev：E IHC IP
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to human Serpin A9 expressed in NSO cells (AAQ89063).
