84-1190-25　RAMP1 (Receptor Activity Modifying Protein1, Receptor Activity Modifying Protein 1 [Precursor], Receptor (G-Protein-coupled) Activity Modifying Protein 1, Calcitonin Receptor-like Receptor Activity Modifying Protein 1, CRLR Activity Modifying Protein 1) 100ug　R1060-03

特徴

• RAMP1 (receptor activity modifying protein 1) is a 14-18kD member of the RAMP family of proteins.
• Variability in MW is due to the absence, or presence, of intramolecular disulfide bonds that form in the ER and Golgi.
• It is expressed on/in neurons, vascular endothelial cells, visceral and vascular smooth muscle cells, mammary epithelium, osteoblasts and cardiomyocytes.
• RAMP1 interacts with CRLR/CLR to form an 84kD noncovalent receptor complex for CGRP and AM, and with CTR to form a 76kD receptor complex for amylin.
• Mature human RAMP1 is a 122 aa nonglycosylated type I transmembrane protein that contains a 91aa extracellular domain (ECD) (aa27-117) plus a ten aa cytoplasmic tail.
• In the ECD, residues 78-90 bind AM, while residues 91-103 bind CGRP.
• In the absence of CRLR and CTR,RAMP1 will form 30-32kD disulfide-linked homodimers in the ER/Golgi.
• Over (aa27-117), human RAMP1 shares 65% aa identity with mouse RAMP1.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:Direct ELISA: 0.5-1ug/mlWestern Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.